DNA-Metallodrugs Interactions Signaled by Electrochemical Biosensors: An Overview

نویسندگان

  • Mauro Ravera
  • Graziana Bagni
  • Marco Mascini
  • Domenico Osella
چکیده

The interaction of drugs with DNA is an important aspect in pharmacology. In recent years, many important technological advances have been made to develop new techniques to monitor biorecognition and biointeraction on solid devices. The interaction between DNA and drugs can cause chemical and conformational modifications and, thus, variation of the electrochemical properties of nucleobases. The propensity of a given compound to interact with DNA is measured as a function of the decrease of guanine oxidation signal on a DNA electrochemical biosensor. Covalent binding at N7 of guanine, electrostatic interactions, and intercalation are the events that this kind of biosensor can detect. In this context, the interaction between a panel of antitumoral Pt-, Ru-, and Ti-based metallodrugs with DNA immobilized on screen-printed electrodes has been studied. The DNA biosensors are used for semiquantitative evaluation of the analogous interaction occurring in the biological environment.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Label-free electrochemical biosensors for food and drug application

In food sector, there is a huge demand for rapid, reliable, user & eco-friendly biosensors to analyse the quality and safety of food products. Biosensor based methodology depends upon the recognition of a specific antigens or receptors by corresponding antibodies, aptamers or high-affinity ligands. The first scientifically commercialised sensors were the electrochemical sensors used for the ana...

متن کامل

Label-free electrochemical biosensors for food and drug application

In food sector, there is a huge demand for rapid, reliable, user & eco-friendly biosensors to analyse the quality and safety of food products. Biosensor based methodology depends upon the recognition of a specific antigens or receptors by corresponding antibodies, aptamers or high-affinity ligands. The first scientifically commercialised sensors were the electrochemical sensors used for the ana...

متن کامل

Advancement in electrochemical DNA-biosensors for GMOs detection: A review

Genetically modified organisms (GMOs) are plants or animals whose genetic make-up has been transformed by recombinant DNA technology, which has new features such as resistance to herbicides, virus and insect. Recently, genetic modification of food products has increased in order to reduce world poverty and hunger and increase food production However, the impact of GMOs on the human health is a ...

متن کامل

Electrochemical Biosensors as a Tool for the Investigation of Dna Structure, Damage and Interaction with Other Molecules

In recent years, a great progress was achieved in the development of electrochemical sensors for DNA sequences, hybridization and damage. Nowadays, electrochemical methods are able to detect DNA at nanomolar concentration. In addition, these methods are suitable for studding both covalent and non-covalent binding interactions between DNA and different small molecules, e.g. drugs or potentially ...

متن کامل

Analysis of Drug-Drug Interactions with Cyclic Voltammetry: An Overview of Relevant Theoretical Models and Recent Experimental Achievements

In this review, we focus on cyclic voltammetry as a reliable electrochemical technique to study mechanisms, kinetics and thermodynamics of various types of drug-drug interactions. While we present and discuss six theoretical models relevant to analyze drug-drug (or drug-DNA) interactions, we also give hints about recent experimental achievements in this field. In addition, we provide the reader...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Bioinorganic Chemistry and Applications

دوره 2007  شماره 

صفحات  -

تاریخ انتشار 2007